Device
Part:BBa_K1718002:Design
Designed by: Daren Kraft and Josephina Hendrix Group: iGEM15_CU_Boulder (2015-09-13)
Genetic Switch for Bxb1 invertase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 55
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 64
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 84
Illegal BsaI.rc site found at 245
Design Notes
The design consists of a terminator to stop transcription from the strong constitutive promoter.
Source
Sequences are based on the Endy experiments.